Wordt geladen...

CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects

Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type V-A CRISPR-Cas anti-phage immune system that can be repurposed for genome editing. Cas12a can bind and cut dsDNA targets with high specificity in vivo, making it an ideal candidate for expanding the arsenal of enzymes used in precise...

Volledige beschrijving

Bewaard in:
Bibliografische gegevens
Gepubliceerd in:J Biol Chem
Hoofdauteurs: Murugan, Karthik, Seetharam, Arun S., Severin, Andrew J., Sashital, Dipali G.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Biochemistry and Molecular Biology 2020
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7186167/
https://ncbi.nlm.nih.gov/pubmed/32161115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.012933
Tags: Voeg label toe
Geen labels, Wees de eerste die dit record labelt!