CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects

Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type V-A CRISPR-Cas anti-phage immune system that can be repurposed for genome editing. Cas12a can bind and cut dsDNA targets with high specificity in vivo, making it an ideal candidate for expanding the arsenal of enzymes used in precise...

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Bibliographic Details
Published in:J Biol Chem
Main Authors: Murugan, Karthik, Seetharam, Arun S., Severin, Andrew J., Sashital, Dipali G.
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7186167/
https://ncbi.nlm.nih.gov/pubmed/32161115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.012933
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