Computer-based Engineering of Thermostabilized Antibody Fragments

We used the molecular modeling program Rosetta to identify clusters of amino acid substitutions in antibody fragments (scFvs and scAbs) that improve global protein stability and resistance to thermal deactivation. Using this methodology, we increased the melting temperature (T(m)) and resistance to...

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Détails bibliographiques
Publié dans:AIChE J
Auteurs principaux: Lee, Jiwon, Der, Bryan S., Karamitros, Christos S., Li, Wenzong, Marshall, Nicholas M., Lungu, Oana I., Miklos, Aleksandr E., Xu, Jianqing, Kang, Tae Hyun, Lee, Chang-Han, Tan, Bing, Hughes, Randall A., Jung, Sang Taek, Ippolito, Gregory C., Gray, Jeffrey J., Zhang, Yan, Kuhlman, Brian, Georgiou, George, Ellington, Andrew D.
Format: Artigo
Langue:Inglês
Publié: 2019
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7181397/
https://ncbi.nlm.nih.gov/pubmed/32336757
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/aic.16864
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