PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells

We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Förster resonance energy transfer...

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Bibliografske podrobnosti
izdano v:Biophys J
Main Authors: Reissaus, Christopher A., Day, Kathleen H., Mirmira, Raghavendra G., Dunn, Kenneth W., Pavalko, Fredrick M., Day, Richard N.
Format: Artigo
Jezik:Inglês
Izdano: The Biophysical Society 2020
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7175463/
https://ncbi.nlm.nih.gov/pubmed/32191861
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.03.003
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