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Measuring Small-molecule Inhibition of Protein Interactions in Live Cells Using FLIM-FRET
This protocol was designed to quantitatively measure small-molecule displacement of proteins in live mammalian cells using fluorescence lifetime imaging microscopy–Förster resonance energy transfer (FLIM-FRET). Tumour cell survival is often dependent on anti-apoptotic proteins, which bind to and inh...
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| Опубликовано в: : | Bio Protoc |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Bio-Protocol
2019
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7853989/ https://ncbi.nlm.nih.gov/pubmed/33654902 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3401 |
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