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PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells
We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Förster resonance energy transfer...
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| Gepubliceerd in: | Biophys J |
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| Hoofdauteurs: | , , , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
The Biophysical Society
2020
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7175463/ https://ncbi.nlm.nih.gov/pubmed/32191861 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.03.003 |
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