PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells

We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Förster resonance energy transfer...

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Bibliografische gegevens
Gepubliceerd in:Biophys J
Hoofdauteurs: Reissaus, Christopher A., Day, Kathleen H., Mirmira, Raghavendra G., Dunn, Kenneth W., Pavalko, Fredrick M., Day, Richard N.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The Biophysical Society 2020
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7175463/
https://ncbi.nlm.nih.gov/pubmed/32191861
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.03.003
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