PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells

We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Förster resonance energy transfer...

詳細記述

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書誌詳細
出版年:Biophys J
主要な著者: Reissaus, Christopher A., Day, Kathleen H., Mirmira, Raghavendra G., Dunn, Kenneth W., Pavalko, Fredrick M., Day, Richard N.
フォーマット: Artigo
言語:Inglês
出版事項: The Biophysical Society 2020
主題:
Article
オンライン･アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7175463/
https://ncbi.nlm.nih.gov/pubmed/32191861
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.03.003
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