PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells

We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Förster resonance energy transfer...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Reissaus, Christopher A., Day, Kathleen H., Mirmira, Raghavendra G., Dunn, Kenneth W., Pavalko, Fredrick M., Day, Richard N.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7175463/
https://ncbi.nlm.nih.gov/pubmed/32191861
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.03.003
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