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Crystal structure of the EcoKMcrA N-terminal domain (NEco): recognition of modified cytosine bases without flipping

EcoKMcrA from Escherichia coli restricts CpG methylated or hydroxymethylated DNA, and may act as a barrier against host DNA. The enzyme consists of a novel N-terminal specificity domain that we term NEco, and a C-terminal catalytic HNH domain. Here, we report that NEco and full-length EcoKMcrA speci...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Slyvka, Anton, Zagorskaitė, Evelina, Czapinska, Honorata, Sasnauskas, Giedrius, Bochtler, Matthias
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7145662/
https://ncbi.nlm.nih.gov/pubmed/31724709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz1017
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