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DNA intercalation without flipping in the specific ThaI–DNA complex
The PD-(D/E)XK type II restriction endonuclease ThaI cuts the target sequence CG/CG with blunt ends. Here, we report the 1.3 Å resolution structure of the enzyme in complex with substrate DNA and a sodium or calcium ion taking the place of a catalytic magnesium ion. The structure identifies Glu54, A...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3025569/ https://ncbi.nlm.nih.gov/pubmed/20861000 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq834 |
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