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Nucleotide flips determine the specificity of the Ecl18kI restriction endonuclease
Restricion endonuclease Ecl18kI is specific for the sequence /CCNGG and cleaves it before the outer C to generate 5 nt 5′-overhangs. It has been suggested that Ecl18kI is evolutionarily related to NgoMIV, a 6-bp cutter that cleaves the sequence G/CCGGC and leaves 4 nt 5′-overhangs. Here, we report t...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2006
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1462983/ https://ncbi.nlm.nih.gov/pubmed/16628220 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7601096 |
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