Nucleotide flips determine the specificity of the Ecl18kI restriction endonuclease

Restricion endonuclease Ecl18kI is specific for the sequence /CCNGG and cleaves it before the outer C to generate 5 nt 5′-overhangs. It has been suggested that Ecl18kI is evolutionarily related to NgoMIV, a 6-bp cutter that cleaves the sequence G/CCGGC and leaves 4 nt 5′-overhangs. Here, we report t...

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Detaylı Bibliyografya
Asıl Yazarlar: Bochtler, Matthias, Szczepanowski, Roman H, Tamulaitis, Gintautas, Grazulis, Saulius, Czapinska, Honorata, Manakova, Elena, Siksnys, Virginijus
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2006
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Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1462983/
https://ncbi.nlm.nih.gov/pubmed/16628220
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7601096
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