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Quantitative real-time imaging of intracellular FRET biosensor dynamics using rapid multi-beam confocal FLIM

Fluorescence lifetime imaging (FLIM) is a quantitative, intensity-independent microscopical method for measurement of diverse biochemical and physical properties in cell biology. It is a highly effective method for measurements of Förster resonance energy transfer (FRET), and for quantification of p...

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Enregistré dans:
Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Levitt, James A., Poland, Simon P., Krstajic, Nikola, Pfisterer, Karin, Erdogan, Ahmet, Barber, Paul R., Parsons, Maddy, Henderson, Robert K., Ameer-Beg, Simon M.
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7083966/
https://ncbi.nlm.nih.gov/pubmed/32198437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-61478-1
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