A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging

We demonstrate diffraction limited multiphoton imaging in a massively parallel, fully addressable time-resolved multi-beam multiphoton microscope capable of producing fluorescence lifetime images with sub-50ps temporal resolution. This imaging platform offers a significant improvement in acquisition...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Poland, Simon P., Krstajić, Nikola, Monypenny, James, Coelho, Simao, Tyndall, David, Walker, Richard J., Devauges, Viviane, Richardson, Justin, Dutton, Neale, Barber, Paul, Li, David Day-Uei, Suhling, Klaus, Ng, Tony, Henderson, Robert K., Ameer-Beg, Simon M.
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354599/
https://ncbi.nlm.nih.gov/pubmed/25780724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.6.000277
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