Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris (Komagataella phaffii): Impact on Cell Physiology and Recombinant Production of Secreted Proteins

High-level expression and secretion of heterologous proteins in yeast cause an increased energy demand, which may result in altered metabolic flux distributions. Moreover, recombinant protein overproduction often results in endoplasmic reticulum (ER) stress and oxidative stress, causing deviations f...

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Vydáno v:Appl Environ Microbiol
Hlavní autoři: Tomàs-Gamisans, Màrius, Andrade, Cristiane Conte Paim, Maresca, Francisco, Monforte, Sergi, Ferrer, Pau, Albiol, Joan
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2020
Témata:
Biotechnology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7054088/
https://ncbi.nlm.nih.gov/pubmed/31757828
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02038-19
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