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Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system

Ganoderma lucidum is an important medicinal mushroom in traditional Chinese medicine. However, the lack of adequate genetic tools has hindered molecular genetic research in and the genetic modification of this species. Here, we report that the presence of an intron is necessary for the efficient exp...

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Detaylı Bibliyografya
Yayımlandı:Microb Biotechnol
Asıl Yazarlar: Liu, Ke, Sun, Bin, You, Hao, Tu, Jun‐Liang, Yu, Xuya, Zhao, Peng, Xu, Jun‐Wei
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: John Wiley and Sons Inc. 2020
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7017817/
https://ncbi.nlm.nih.gov/pubmed/31958883
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13534
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