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Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts

We present a new approach to edit both mitochondrial and chloroplast genomes. Organelles have been considered off-limits to CRISPR due to their impermeability to most RNA and DNA. This has prevented applications of Cas9/gRNA-mediated genome editing in organelles while the tool has been widely used f...

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Détails bibliographiques
Publié dans:PeerJ
Auteurs principaux: Yoo, Byung-Chun, Yadav, Narendra S., Orozco, Emil M., Sakai, Hajime
Format: Artigo
Langue:Inglês
Publié: PeerJ Inc. 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6951285/
https://ncbi.nlm.nih.gov/pubmed/31934513
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.8362
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