Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid

While CRISPR-Cas9-mediated genome editing has transformed yeast research, current plasmids and cassettes for Cas9 and guide-RNA expression are species specific. CRISPR tools that function in multiple yeast species could contribute to the intensifying research on non-conventional yeasts. A plasmid ca...

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Detalhes bibliográficos
Publicado no:FEMS Yeast Res
Main Authors: Juergens, Hannes, Varela, Javier A, Gorter de Vries, Arthur R, Perli, Thomas, Gast, Veronica J M, Gyurchev, Nikola Y, Rajkumar, Arun S, Mans, Robert, Pronk, Jack T, Morrissey, John P, Daran, Jean-Marc G
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6018904/
https://ncbi.nlm.nih.gov/pubmed/29438517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsyr/foy012
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