Cas9 gRNA engineering for genome editing, activation and repression

We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits wit...

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Publicado en:Nat Methods
Autores principales: Kiani, Samira, Chavez, Alejandro, Tuttle, Marcelle, Hall, Richard N, Chari, Raj, Ter-Ovanesyan, Dmitry, Qian, Jason, Pruitt, Benjamin W, Beal, Jacob, Vora, Suhani, Buchthal, Joanna, Kowal, Emma J K, Ebrahimkhani, Mohammad R, Collins, James J, Weiss, Ron, Church, George
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666719/
https://ncbi.nlm.nih.gov/pubmed/26344044
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3580
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