Cas9 gRNA engineering for genome editing, activation and repression

We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits wit...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nat Methods
Hauptverfasser: Kiani, Samira, Chavez, Alejandro, Tuttle, Marcelle, Hall, Richard N, Chari, Raj, Ter-Ovanesyan, Dmitry, Qian, Jason, Pruitt, Benjamin W, Beal, Jacob, Vora, Suhani, Buchthal, Joanna, Kowal, Emma J K, Ebrahimkhani, Mohammad R, Collins, James J, Weiss, Ron, Church, George
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
Schlagworte:
Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666719/
https://ncbi.nlm.nih.gov/pubmed/26344044
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3580
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge