Cargando...
Cas9 gRNA engineering for genome editing, activation and repression
We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits wit...
Guardado en:
| Publicado en: | Nat Methods |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2015
|
| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4666719/ https://ncbi.nlm.nih.gov/pubmed/26344044 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3580 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|