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CRISPR transcriptional repression devices and layered circuits in mammalian cells
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of...
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Publicado no: | Nat Methods |
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Main Authors: | , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2014
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4228775/ https://ncbi.nlm.nih.gov/pubmed/24797424 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2969 |
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