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CRISPR transcriptional repression devices and layered circuits in mammalian cells

A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of...

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Vydáno v:Nat Methods
Hlavní autoři: Kiani, Samira, Beal, Jacob, Ebrahimkhani, Mohammad R, Huh, Jin, Hall, Richard N, Xie, Zhen, Li, Yinqing, Weiss, Ron
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4228775/
https://ncbi.nlm.nih.gov/pubmed/24797424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2969
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