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gRNA validation for wheat genome editing with the CRISPR-Cas9 system
BACKGROUND: The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assess...
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| Publicado no: | BMC Biotechnol |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6829922/ https://ncbi.nlm.nih.gov/pubmed/31684940 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-019-0565-z |
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