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gRNA validation for wheat genome editing with the CRISPR-Cas9 system

BACKGROUND: The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assess...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:BMC Biotechnol
Main Authors: Arndell, Taj, Sharma, Niharika, Langridge, Peter, Baumann, Ute, Watson-Haigh, Nathan S., Whitford, Ryan
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6829922/
https://ncbi.nlm.nih.gov/pubmed/31684940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-019-0565-z
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