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An in vitro platform for engineering and harnessing modular polyketide synthases

To harness the synthetic power of modular polyketide synthases (PKSs), many aspects of their biochemistry must be elucidated. A robust platform to study these megadalton assembly lines has not yet been described. Here, we in vitro reconstitute the venemycin PKS, a short assembly line that generates...

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Publié dans:Nat Commun
Auteurs principaux: Miyazawa, Takeshi, Hirsch, Melissa, Zhang, Zhicheng, Keatinge-Clay, Adrian T.
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6941969/
https://ncbi.nlm.nih.gov/pubmed/31900404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-13811-0
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