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Bi-allelic Homology-Directed Repair with Helper-Dependent Adenoviruses
We describe a strategy to achieve footprintless bi-allelic homology-directed repair (HDR) using helper-dependent adenoviruses (HDAds). This approach utilizes two HDAds to deliver the donor DNA. These two HDAds are identical except for their selectable marker. One expresses the puromycin N-acetyltran...
Gespeichert in:
| Veröffentlicht in: | Mol Ther Methods Clin Dev |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society of Gene & Cell Therapy
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6923503/ https://ncbi.nlm.nih.gov/pubmed/31890728 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtm.2019.10.003 |
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