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Production of CRISPR/Cas9-Mediated Self-Cleaving Helper-Dependent Adenoviruses
Prolonged expression of CRISPR/Cas9 raises concerns about off-target cleavage, cytotoxicity, and immune responses. To address these issues, we have developed a system to produce helper-dependent adenoviruses that express CRISPR/Cas9 to direct cleavage of the vectors’ own genome after transduction of...
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| Publicado no: | Mol Ther Methods Clin Dev |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society of Gene & Cell Therapy
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6506437/ https://ncbi.nlm.nih.gov/pubmed/31080846 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtm.2019.04.003 |
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