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Bi-allelic Homology-Directed Repair with Helper-Dependent Adenoviruses

We describe a strategy to achieve footprintless bi-allelic homology-directed repair (HDR) using helper-dependent adenoviruses (HDAds). This approach utilizes two HDAds to deliver the donor DNA. These two HDAds are identical except for their selectable marker. One expresses the puromycin N-acetyltran...

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Bibliografski detalji
Izdano u:Mol Ther Methods Clin Dev
Glavni autori: Palmer, Donna J., Turner, Dustin L., Ng, Philip
Format: Artigo
Jezik:Inglês
Izdano: American Society of Gene & Cell Therapy 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6923503/
https://ncbi.nlm.nih.gov/pubmed/31890728
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtm.2019.10.003
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