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Using Gene Editing to Establish a Safeguard System for Pluripotent Stem-Cell-Based Therapies

A major challenge in using human pluripotent stem cells (hPSCs) in therapy is the risk of teratoma formation due to contaminating undifferentiated stem cells. We used CRISPR-Cas9 for in-frame insertion of a suicide gene, iC9, into the endogenous SOX2 locus in human embryonic stem cell (ESC) line H1...

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Detalhes bibliográficos
Publicado no:iScience
Main Authors: Wu, Youjun, Chang, Tammy, Long, Yan, Huang, He, Kandeel, Fouad, Yee, Jiing-Kuan
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6909005/
https://ncbi.nlm.nih.gov/pubmed/31821946
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2019.11.038
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