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An efficient protein production system via gene amplification on a human artificial chromosome and the chromosome transfer to CHO cells

Gene amplification methods play a crucial role in establishment of cells that produce high levels of recombinant protein. However, the stability of such cell lines and the level of recombinant protein produced continue to be suboptimal. Here, we used a combination of a human artificial chromosome (H...

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Bibliografske podrobnosti
izdano v:Sci Rep
Main Authors: Ohira, Takahito, Miyauchi, Koichi, Uno, Narumi, Shimizu, Noriaki, Kazuki, Yasuhiro, Oshimura, Mitsuo, Kugoh, Hiroyuki
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6861226/
https://ncbi.nlm.nih.gov/pubmed/31740706
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-53116-2
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