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An efficient protein production system via gene amplification on a human artificial chromosome and the chromosome transfer to CHO cells
Gene amplification methods play a crucial role in establishment of cells that produce high levels of recombinant protein. However, the stability of such cell lines and the level of recombinant protein produced continue to be suboptimal. Here, we used a combination of a human artificial chromosome (H...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6861226/ https://ncbi.nlm.nih.gov/pubmed/31740706 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-53116-2 |
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