Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Microenvironment evaluation of intact tissue for analysis of cell infiltration and spatial organization are essential in understanding the complexity of disease processes. The principle techniques used in the past include immunohistochemistry (IHC) and immunofluorescence (IF) which enable visualizat...

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteurs: Lazarus, Jenny, Akiska, Yagiz, Lanfranca, Mirna Perusina, Delrosario, Lawrence, Sun, Lei, Long, Daniel, Shi, Jiaqi, Crawford, Howard, Di Magliano, Marina P., Zou, Weiping, Frankel, Timothy
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2019
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6786767/
https://ncbi.nlm.nih.gov/pubmed/31403624
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59915
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