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High‐throughput single‐cell sequencing of paired TCRα and TCRβ genes for the direct expression‐cloning and functional analysis of murine T‐cell receptors

Precise clonal and functional assessments of the T cell receptor (TCR) repertoire diversity require paired TCRα and TCRβ gene sequence information at monoclonal level. However, available single‐cell strategies are typically limited in throughput and often do not provide full‐length DNA templates for...

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Bibliographic Details
Published in:Eur J Immunol
Main Authors: Ludwig, Julia, Huber, Ann‐Kathrin, Bartsch, Ilka, Busse, Christian E., Wardemann, Hedda
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6767390/
https://ncbi.nlm.nih.gov/pubmed/31017295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/eji.201848030
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