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High‐throughput single‐cell sequencing of paired TCRα and TCRβ genes for the direct expression‐cloning and functional analysis of murine T‐cell receptors

Precise clonal and functional assessments of the T cell receptor (TCR) repertoire diversity require paired TCRα and TCRβ gene sequence information at monoclonal level. However, available single‐cell strategies are typically limited in throughput and often do not provide full‐length DNA templates for...

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Detalhes bibliográficos
Publicado no:Eur J Immunol
Main Authors: Ludwig, Julia, Huber, Ann‐Kathrin, Bartsch, Ilka, Busse, Christian E., Wardemann, Hedda
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6767390/
https://ncbi.nlm.nih.gov/pubmed/31017295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/eji.201848030
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