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Targeted DNA transposition in vitro using a dCas9-transposase fusion protein

Homology-directed genome engineering is limited by transgene size. Although DNA transposons are more efficient with large transgenes, random integrations are potentially mutagenic. Here we present an in vitro mechanistic study that demonstrates efficient Cas9 targeting of the mariner transposon Hsma...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Bhatt, Shivam, Chalmers, Ronald
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6735945/
https://ncbi.nlm.nih.gov/pubmed/31429873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz552
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