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DNA targeting by Clostridium cellulolyticum CRISPR–Cas9 Type II-C system
Type II CRISPR–Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a relatively big size, imperfect specificity and restrict...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7038990/ https://ncbi.nlm.nih.gov/pubmed/31943070 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz1225 |
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