Microhomologies are prevalent at Cas9-induced larger deletions

The CRISPR system is widely used in genome editing for biomedical research. Here, using either dual paired Cas9(D10A) nickases or paired Cas9 nuclease we characterize unintended larger deletions at on-target sites that frequently evade common genotyping practices. We found that unintended larger del...

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Udgivet i:Nucleic Acids Res
Main Authors: Owens, Dominic D G, Caulder, Adam, Frontera, Vincent, Harman, Joe R, Allan, Alasdair J, Bucakci, Akin, Greder, Lucas, Codner, Gemma F, Hublitz, Philip, McHugh, Peter J, Teboul, Lydia, de Bruijn, Marella F T R
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2019
Fag:
Genome Integrity, Repair and Replication
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6698657/
https://ncbi.nlm.nih.gov/pubmed/31127293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz459
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