Microhomologies are prevalent at Cas9-induced larger deletions

The CRISPR system is widely used in genome editing for biomedical research. Here, using either dual paired Cas9(D10A) nickases or paired Cas9 nuclease we characterize unintended larger deletions at on-target sites that frequently evade common genotyping practices. We found that unintended larger del...

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Detaylı Bibliyografya
Yayımlandı:Nucleic Acids Res
Asıl Yazarlar: Owens, Dominic D G, Caulder, Adam, Frontera, Vincent, Harman, Joe R, Allan, Alasdair J, Bucakci, Akin, Greder, Lucas, Codner, Gemma F, Hublitz, Philip, McHugh, Peter J, Teboul, Lydia, de Bruijn, Marella F T R
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 2019
Konular:
Genome Integrity, Repair and Replication
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6698657/
https://ncbi.nlm.nih.gov/pubmed/31127293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz459
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