Microhomologies are prevalent at Cas9-induced larger deletions

The CRISPR system is widely used in genome editing for biomedical research. Here, using either dual paired Cas9(D10A) nickases or paired Cas9 nuclease we characterize unintended larger deletions at on-target sites that frequently evade common genotyping practices. We found that unintended larger del...

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Bibliografski detalji
Izdano u:Nucleic Acids Res
Glavni autori: Owens, Dominic D G, Caulder, Adam, Frontera, Vincent, Harman, Joe R, Allan, Alasdair J, Bucakci, Akin, Greder, Lucas, Codner, Gemma F, Hublitz, Philip, McHugh, Peter J, Teboul, Lydia, de Bruijn, Marella F T R
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2019
Teme:
Genome Integrity, Repair and Replication
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6698657/
https://ncbi.nlm.nih.gov/pubmed/31127293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz459
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