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Functional Analysis of Human BRCA2 Variants Using a Mouse Embryonic Stem Cell-Based Assay

We describe here a comprehensive and reliable assay to test the functional significance of variants of unknown clinical significance (VUS) identified in the human breast cancer susceptibility gene, BRCA2. The assay is based on the ability of human BRCA2 to complement the loss of endogenous Brca2 in...

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Publicat a:Methods Mol Biol
Autors principals: Kuznetsov, Sergey G., Chang, Suhwan, Sharan, Shyam K.
Format: Artigo
Idioma:Inglês
Publicat: 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6669890/
https://ncbi.nlm.nih.gov/pubmed/20721749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-759-4_16
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