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Impact of sequencing depth and technology on de novo RNA-Seq assembly

BACKGROUND: RNA-Seq data is inherently nonuniform for different transcripts because of differences in gene expression. This makes it challenging to decide how much data should be generated from each sample. How much should one spend to recover the less expressed transcripts? The sequencing technolog...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Patterson, Jordan, Carpenter, Eric J., Zhu, Zhenzhen, An, Dan, Liang, Xinming, Geng, Chunyu, Drmanac, Radoje, Wong, Gane Ka-Shu
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6651908/
https://ncbi.nlm.nih.gov/pubmed/31337347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-019-5965-x
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