Impact of sequencing depth and technology on de novo RNA-Seq assembly

BACKGROUND: RNA-Seq data is inherently nonuniform for different transcripts because of differences in gene expression. This makes it challenging to decide how much data should be generated from each sample. How much should one spend to recover the less expressed transcripts? The sequencing technolog...

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Udgivet i:BMC Genomics
Main Authors: Patterson, Jordan, Carpenter, Eric J., Zhu, Zhenzhen, An, Dan, Liang, Xinming, Geng, Chunyu, Drmanac, Radoje, Wong, Gane Ka-Shu
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2019
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6651908/
https://ncbi.nlm.nih.gov/pubmed/31337347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-019-5965-x
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