Impact of sequencing depth and technology on de novo RNA-Seq assembly

BACKGROUND: RNA-Seq data is inherently nonuniform for different transcripts because of differences in gene expression. This makes it challenging to decide how much data should be generated from each sample. How much should one spend to recover the less expressed transcripts? The sequencing technolog...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:BMC Genomics
Main Authors: Patterson, Jordan, Carpenter, Eric J., Zhu, Zhenzhen, An, Dan, Liang, Xinming, Geng, Chunyu, Drmanac, Radoje, Wong, Gane Ka-Shu
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2019
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6651908/
https://ncbi.nlm.nih.gov/pubmed/31337347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-019-5965-x
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