Impact of sequencing depth and technology on de novo RNA-Seq assembly

BACKGROUND: RNA-Seq data is inherently nonuniform for different transcripts because of differences in gene expression. This makes it challenging to decide how much data should be generated from each sample. How much should one spend to recover the less expressed transcripts? The sequencing technolog...

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Vydáno v:BMC Genomics
Hlavní autoři: Patterson, Jordan, Carpenter, Eric J., Zhu, Zhenzhen, An, Dan, Liang, Xinming, Geng, Chunyu, Drmanac, Radoje, Wong, Gane Ka-Shu
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2019
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Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6651908/
https://ncbi.nlm.nih.gov/pubmed/31337347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-019-5965-x
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