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In vitro reconstitution of yeast RNA polymerase II transcription initiation with high efficiency
Transcription initiation can be reconstituted from highly purified general transcription factors (GTFs), RNA polymerase II (pol II), and promoter DNA. However, earlier biochemical reconstitution systems had a serious technical limitation, namely very poor initiation efficiency. Due to the poor effic...
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| Izdano u: | Methods |
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| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2019
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6628718/ https://ncbi.nlm.nih.gov/pubmed/30905750 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.03.016 |
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