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In vitro reconstitution of yeast RNA polymerase II transcription initiation with high efficiency

Transcription initiation can be reconstituted from highly purified general transcription factors (GTFs), RNA polymerase II (pol II), and promoter DNA. However, earlier biochemical reconstitution systems had a serious technical limitation, namely very poor initiation efficiency. Due to the poor effic...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Fujiwara, Rina, Murakami, Kenji
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6628718/
https://ncbi.nlm.nih.gov/pubmed/30905750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.03.016
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