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In vitro reconstitution of yeast RNA polymerase II transcription initiation with high efficiency
Transcription initiation can be reconstituted from highly purified general transcription factors (GTFs), RNA polymerase II (pol II), and promoter DNA. However, earlier biochemical reconstitution systems had a serious technical limitation, namely very poor initiation efficiency. Due to the poor effic...
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| Publicado no: | Methods |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6628718/ https://ncbi.nlm.nih.gov/pubmed/30905750 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.03.016 |
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