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In vitro reconstitution of yeast RNA polymerase II transcription initiation with high efficiency
Transcription initiation can be reconstituted from highly purified general transcription factors (GTFs), RNA polymerase II (pol II), and promoter DNA. However, earlier biochemical reconstitution systems had a serious technical limitation, namely very poor initiation efficiency. Due to the poor effic...
Guardat en:
| Publicat a: | Methods |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2019
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6628718/ https://ncbi.nlm.nih.gov/pubmed/30905750 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.03.016 |
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