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In vitro reconstitution of yeast RNA polymerase II transcription initiation with high efficiency

Transcription initiation can be reconstituted from highly purified general transcription factors (GTFs), RNA polymerase II (pol II), and promoter DNA. However, earlier biochemical reconstitution systems had a serious technical limitation, namely very poor initiation efficiency. Due to the poor effic...

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Vydáno v:Methods
Hlavní autoři: Fujiwara, Rina, Murakami, Kenji
Médium: Artigo
Jazyk:Inglês
Vydáno: 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6628718/
https://ncbi.nlm.nih.gov/pubmed/30905750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.03.016
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