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Efficient reconstitution of transcription elongation complexes for single-molecule studies of eukaryotic RNA polymerase II
Single-molecule studies of RNA polymerase II (RNAP II) require high yields of transcription elongation complexes (TECs) with long DNA tethers upstream and downstream of the TEC. Here we report on a robust system to reconstitute both yeast and mammalian RNAP II with an efficiency of ~80% into TECs th...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Landes Bioscience
2012
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3616084/ https://ncbi.nlm.nih.gov/pubmed/22771949 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/trns.20269 |
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