Efficient reconstitution of transcription elongation complexes for single-molecule studies of eukaryotic RNA polymerase II

Single-molecule studies of RNA polymerase II (RNAP II) require high yields of transcription elongation complexes (TECs) with long DNA tethers upstream and downstream of the TEC. Here we report on a robust system to reconstitute both yeast and mammalian RNAP II with an efficiency of ~80% into TECs th...

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Hauptverfasser: Palangat, Murali, Larson, Matthew H., Hu, Xiaopeng, Gnatt, Averell, Block, Steven M., Landick, Robert
Format: Artigo
Sprache:Inglês
Veröffentlicht: Landes Bioscience 2012
Schlagworte:
Methods Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3616084/
https://ncbi.nlm.nih.gov/pubmed/22771949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/trns.20269
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