Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos

A substantial number of mouse genes, about 25%, are embryonically lethal when knocked out. Using current genetic tools, such as the CRISPR-Cas9 system, it is difficult—or even impossible—to produce viable mice with heritable embryonically lethal mutations. Here, we establish a one-step method for mi...

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Bibliographic Details
Published in:Nat Commun
Main Authors: Wu, Yi, Zhang, Jing, Peng, Boya, Tian, Dan, Zhang, Dong, Li, Yang, Feng, Xiaoyu, Liu, Jinghao, Li, Jun, Zhang, Teng, Liu, Xiaoyong, Lu, Jing, Chen, Baian, Wang, Songlin
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6599060/
https://ncbi.nlm.nih.gov/pubmed/31253768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-10748-2
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