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Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift
Measuring protein/DNA interactions that have apparent binding affinity constants in the low picomolar range presents a unique experimental challenge. To probe the sequence specificity of telomere-binding proteins, our lab has developed an electrophoretic mobility shift assay protocol that allows for...
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| Published in: | Methods Mol Biol |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6557633/ https://ncbi.nlm.nih.gov/pubmed/30426430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8793-1_29 |
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