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Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift
Measuring protein/DNA interactions that have apparent binding affinity constants in the low picomolar range presents a unique experimental challenge. To probe the sequence specificity of telomere-binding proteins, our lab has developed an electrophoretic mobility shift assay protocol that allows for...
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| Publicat a: | Methods Mol Biol |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2019
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6557633/ https://ncbi.nlm.nih.gov/pubmed/30426430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8793-1_29 |
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