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Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment

Genomic DNA in eukaryotes is organized into chromatin through association with core histones to form nucleosomes, each distinguished by their DNA sequences and histone variants. Here, we used a single-chain antibody fragment (scFv) derived from the anti-nucleosome antibody mAb PL2-6 to stabilize hum...

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Bibliografiske detaljer
Udgivet i:Nat Commun
Main Authors: Zhou, Bing-Rui, Yadav, K. N. Sathish, Borgnia, Mario, Hong, Jingjun, Cao, Baohua, Olins, Ada L., Olins, Donald E., Bai, Yawen, Zhang, Ping
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6534667/
https://ncbi.nlm.nih.gov/pubmed/31127102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-10247-4
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