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Generating CRISPR/Cas9-Derived Mutant Mice by Zygote Cytoplasmic Injection Using an Automatic Microinjector

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) assisted generation of mutant animals has become the method of choice for the elucidation of gene function in development and disease due to the shortened timelines for generation of a desired mutant, the ease...

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Bibliografiske detaljer
Udgivet i:Methods Protoc
Main Authors: Doe, Brendan, Brown, Ellen, Boroviak, Katharina
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6526459/
https://ncbi.nlm.nih.gov/pubmed/31164552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps1010005
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