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Generating CRISPR/Cas9-Derived Mutant Mice by Zygote Cytoplasmic Injection Using an Automatic Microinjector

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) assisted generation of mutant animals has become the method of choice for the elucidation of gene function in development and disease due to the shortened timelines for generation of a desired mutant, the ease...

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Dettagli Bibliografici
Pubblicato in:Methods Protoc
Autori principali: Doe, Brendan, Brown, Ellen, Boroviak, Katharina
Natura: Artigo
Lingua:Inglês
Pubblicazione: MDPI 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6526459/
https://ncbi.nlm.nih.gov/pubmed/31164552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps1010005
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